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It differs from native IGF-1 in that it possesses an arginine instead of a glutamic acid at the third position in its amino acid sequence, and also has an additional 13 amino acids at its N-terminus (MFPAMPLLSLFVN), for a total of 83 amino acids relative to the 70 of native IGF-1

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HGH (human growth hormone) is the actual hormone, supplied directly

Erythrocytes were then washed three times at 1000 g for 5 min at 4 C with an isotonic buffer (145 mM NaCl, 5 mM glucose, and 20 mM HEPES

Source: Jastreboff et al., NEJM 2022 (SURMOUNT-1)