Vitamin E and glutathione are required for preservation of microsomal glutathione [PMID: 2371233] Laboratory literature summary: Vitamin E and glutathione are required for preservation of microsomal glutathione S-transferase from oxidative stress in microsomes
(2022) 23:e54685
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For the preparation of nuclear extracts, frozen liver pieces were homogenized in homogenization buffer (20 mM HEPES, 70 mM sucrose, 220 mM Mannitol, 2 mM EDTA, 0.5 mg/ml BSA, 0.1 mM PMSF, 1.0 mM DTT, pH 7.5)
[0009] Amines are organic compounds and functional groups that contain a basic nitrogen atom with a lone pair