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Cox HD, Smeal SJ, Hughes CM, Cox JE, Eichner D

This can reduce the amount of usable peptide in the solution and affect results

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Nevertheless, use of these chemokines as prognostic factors for ALS was not investigated in this study, as the chemokines were analyzed in CSF and sera from ALS patients only once at early stage of disease (281 days from first symptoms to diagnosis)

They were then blocked with 5% bovine serum albumin (BSA) in PBS at 37 C for 1 h to prevent non-specific antibody binding

Lastly, there was a 70% increase in the production of collagen by these cells