Images were captured using a camera (Evolve 512 Delta, Photometrics), acquisitions were saved and data were logged using Metamorph software (Molecular Devices)
The two anti-glucagon antibodies were mixed in a 1:1 donor (cryptate)/acceptor (d2) ratio in the glucagon assay detection buffer (Cisbio Bioassays) and 10 L of this glucagon antibody mix was added to each well
249 Nuclear STAT3-Y705 activates glycolysis and fatty acid oxidation (FAO) genes, while mitochondrial STAT3-S727 and acetylated STAT3-K685 impair oxidative phosphorylation by destabilizing electron transport chain (ETC) complexes, supporting leukemia stem cell dormancy and persistence
Pro Tip: This pairs perfectly with your pre-workout on training days, or take it standalone on rest days to maintain muscle and cognitive saturation
To stay safe, always receive glutathione IVs from a licensed and reputable provider
If you carry one of these MTHFR variants, especially two copies, it is worth discussing a homocysteine test with your physician, but dont stress