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In affinity chromatography you take a mix of proteins and other cellular content and flow it through a resin (little beads) coated with something the affinity tag has specific affinity for (e.g

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Oxidative stress (e.g., hypoxia and ischemia/reperfusion) induces autophagy to degrade damaged organelles and proteins, while autophagy itself regulates redox balance by clearing oxidized products (44)