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This section will explain why the thigh is a popular choice, provide tips for proper technique, and address common concerns about injecting into the thigh

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For the extraction of total RNA, tissue samples were pulverized using GenoGrinder (ColeParmer), then homogenized in Tri Reagent (Molecular Research Center or Ambion by Life Technologies) using a MagNa Lyser or TissueLyser II system (Qiagen)

Prioritizing health-promoting habits like regular meals, joyful movement, sleep and stress management over appearance-based goals