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The addition of energy expenditure enhancement via glucagon agonism addresses a limitation inherent to GLP-1-only approaches, which reduce energy intake but do not meaningfully increase energy output

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These cells were subsequently washed with Ca 2+ and Mg 2+ -containing PBS, followed by the application of 30 M DCFDA and incubation at 37 C for 30 min in a dark incubator

Figure 4 summarises the 3-year adverse events

The key challenges include high production costs, low yield, high voltage requirements, clogging, and the use of toxic materials in complex techniques

He L, et al