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Intracellular cAMP was measured using the YFP-Epac-Rluc CAMYEL sensor 39

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There's no requirement to separate them by a specific number of hours, but using different injection sites is standard practice

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reviewed the evidence as to whether any of the available forms of B12 are superior, and concluded that all forms can be converted to the active methyl- and adenosyl-B12 forms, and that there was not sufficient evidence to suggest that any form was superior in terms of bioavailability, biochemical effects or clinical efficacy