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Transwell chambers were assayed for cell invasion ability by applying 100 L of DMEM-diluted Matrigel gel (3:7 dilution) to the chambers to be checked and incubated at 37 C for 30 min to form a gel, then pre-treated MIA PaCa-2 and PANC-1 cells were resuspended with DMEM and spread in small chambers at 50000/well, and the lower chamber was placed in 20% FBS DMEM, incubated at 37C 5% CO 2 for 48 hours
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11) Kidney Health Oxidative stress in the kidneys can cause mild kidney problems or even full-blown kidney failure, depending on its severity
Peroxyl radical scavenging capacity Peroxyl radical scavenging capacity was measured fluorometrically as a proportion of antioxidant consumption relative to that of Trolox, as described previously.20 Determination of heme oxygenase enzyme activity PA-TU-8902 cells were incubated for 24 h with tested therapeutics
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