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Luminal A is the most common molecular subtype of breast cancer, accounting for approximately 50% of invasive breast cancer cases [2], and is characterized as estrogen receptor-positive (ER+), progesterone receptor-positive (PR+), HER2/Neu-negative, with low Ki-67 expression [3]
5.16 RNA isolation, reverse transcription and quantitative real-time PCR For mRNA isolation from primary IEC or organoids, the NucleoSpin RNAII kit (Macherey-Nagel) was used according to the manufacturers instructions