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Liver paraffin sections (5 mm) were prepared and stained with hematoxylin and eosin (H&E) by the Department of Pathology at the University of Colorado Denver using standard procedures

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Next, we introduced multiple mutations in HGF, including point mutations (W321R, E336R, E361R, R373E, and Y376A), double mutation (W321R/Y376A), and triple mutation (W321R/E361R/Y376A), designed to weaken the interface III