Measurement of bound sulfane sulfur A whole brain homogenates were prepared with 9 volumes of ice-cold buffer consisting of 10 mM potassium phosphate (pH 7.4), 1% TritonX-100, 10 mM hydroxylamine, which was used to suppress the activity of PLP-dependent enzymes involved in enzymatic H 2 S production, and protease inhibitor cocktail complete (Roche Diagnostics, Mannheim, Germany) using a Potter type glass homogenizer with a Teflon pestle (1,500 rpm, 10 strokes)
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Anger B, Bockman R, Andreeff M , Erlandson R, Jhanwar S, Kameya T, Saigo P, Wright W, Beattie EJ, Jr, Oettgen HF, Old LJ
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