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At the completion of the incubation, the cells were washed three times with ice-cold phosphate-buffered saline (PBS), and then, the fluorescence was measured by fluorescence microscopy at an emission wavelength of 460 nm using an excitation wavelength of 360 nm for the DCF fluorophore
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It has been associated with complications from diabetic retinopathy
The collected synchronized zebrafish embryos were arrayed by pipette into a 96-well plate, three embryos per well with 200 l embryo medium
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