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To the other portion, add 2.0 mL of standard sodium chloride solution (prepared by dissolving 0.126 g of sodium chloride, dried at 105C for 2 hours, in water in a 1000 mL volumetric flask and diluting to volume to obtain a solution containing 50 g of sodium per 1 mL), dilute with water to volume, mix well, and use as the reference solution

Understanding Lyophilized Peptides Most research peptides are supplied as lyophilized powders

We use the Charles River Endosafe PTS system to test for bacterial endotoxins following USP guidelines: The following are suitability parameters that verify the system was working properly and the sample prep dilution is appropriate for accurate results

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Add 1 mL of 1 mol/L hydrochloric acid solution, mix well, and then add 2 drops of ammonium reineckate TS

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