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The DNA sequence spanning the CBS gene ARE motif located 4970 bp upstream of the transcript start site and its mutants were cloned into a GFP expressing reporter pEGFP-N1 (PT3054, Clontech) with sequence specific primers (Supplementary Table 1)

The cfDNA and the immune complex, containing citrullinated protein, incite immune cells secretion of superabundant inflammatory factors [11, 12]

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