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In 1964, the development of insulin radioimmunoassay methods led to the discovery of the incretin effect, which demonstrated that oral glucose intake induces higher insulin secretion compared to intravenous (i.v.) glucose [3,4]

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Measure the absorbance at 412nm, and calculate the amount of GSH

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g , top left, Experimental setting in which histidine was added at increasing mM concentrations to de-iniDCs prior to co-culture