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Materials and Methods Cell culture and reagents Dissociated cortical neuron cultures from Sprague-Dawley rats were prepared as described previously (62)
In our opinion, the increase in hydrogen sulfide production in glutathione-treated old animals was due to an increase in the expression of 3-MST (by 25%), or probably the activity of this mitochondrial enzyme due to an excess of the substrate L-cysteine, which is common for the synthesis of this gaseous mediator and glutathione
Glutathione is a naturally occurring substance and is generally well-tolerated by most people
Free radicals in the physiological control of cell function
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