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The annealed DNA was then serially mixed in a 3:1 molar ratio with concentrated Pol catalytic domain (Val235-Trp575, 12.74 mg/mL)followed by addition of the incoming nonhydrolyzable dUMPNPP nucleotide (0.91 mM final concentration)
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The reasons are numerous and include poor gut health (impaired absorption), high stress (increased cortisol), obesity, inadequate consumption of fat, aging and inflammation
doi:10.3390/antiox12071445 74
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