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It should never be shaken, since agitation promotes aggregation and produces foaming that makes accurate handling difficult
Critical role of Nrf2 in oxidative stress-induced retinal ganglion cell death
Tissue samples: Homogenization lysis was performed using a suitable buffer (PBS, saline, etc.), where a ratio of 1 to 9 (0.1 g: 0.9 mL) of tissue to saline was sufficient
The most common reported issue is mild irritation at the injection site
Thinner individuals use 45 degrees
PMID 10220143
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