Severity of Deficiency The speed of treatment depends on your current B12 deficiency level

Step-by-step reconstitution process Supplies needed: 50mg GHK-Cu vial (lyophilized powder) 5ml bacteriostatic water 3ml or 5ml syringe Alcohol wipes Reconstitution steps: Clean workspace Wash hands thoroughly Wipe vials with alcohol Draw bacteriostatic water Draw 5ml into syringe Remove air bubbles Add water to GHK-Cu Insert needle through rubber stopper Slowly inject water down side of vial (not directly on powder) Aim stream at glass wall, not powder Let dissolve Remove syringe Swirl gently (don't shake) Let sit 1-3 minutes Swirl again until completely clear Inspect solution Should be clear (slightly blue tint normal) No particles or cloudiness If not clear, swirl more Label vial Write concentration (10mg/ml) Write date reconstituted Write expiration (30 days) Refrigerate immediately Store at 2-8C Protect from light Use within 30 days See our peptide storage guide , how long reconstituted peptides last in fridge , and lyophilized vs liquid peptides

The choice depends on several factors, such as the injection dose, frequency, and medical equipment
It is naturally found in a variety of animal foods and is available as a dietary supplement or through vitamin injections
The correct syringe draw depends on the final concentration, not only the vial amount
If your levels were very low, the change can feel more obvious during those first couple of days