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Mycobacterium smegmatis (MS) was used as the expression system to produce active soluble recombinant PLC from Mtb that was expressed at very low levels, therefore the biochemical properties and the substrate specificity of Mtbs PLCs are yet to be determined (Johansen et al., 1996)

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The analyses were performed using a BEH HILIC 1.7 m 1.0 100 mm Acquity UPLC column (Waters)

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