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Int J Mol Sci (2021) 22(7):3330

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Miniaturized rodent LMS were cultured in a 12-well plate format in M199 (P04-07050, Pan) with 3% penicillin/streptomycin (15140122, Thermo Fisher Scientific) and 1:100 insulin-transferrin-selenium (P07-03210, PAN-Biotech, Aidenbach, Germany) at 37 C and 5% CO 2 and stimulated with the modified IONOPTIX C-Pace (pulse duration 5 ms, amplitude 6 V, frequency 0.2 Hz) as published before [29]