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Those who scanned quarterly lost 10 % over the same period

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Sections were treated with Vectastain ABC reagent (Vector Laboratories, PK-4000, California, USA)for 30 min and immunoreactivity was visualized using diaminobenzidine (DAB) (abcam, ab64238, Cambridge, UK) as chromogen and hematoxylin (Merck, 51728-2, Darmstadt, Germany) as counterstain

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