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MDA, 4-HNE, GSH and GSSG were assessed by ELISA kits
J Chromatogr B
The takeaway: after reconstitution, you should plan to use the solution within one month or less , ideally sooner, under refrigeration (2-8 C) and avoiding freezing/thawing cycles if possible
It is contained in many foods, especially fruits, vegetables and meat
For viability, 7-aminoactinomycin D (7AAD) (Thermo fisher A1310) or Zombie NIR (BioLegend 423106) diluted 1:3000 was stained concurrently with antibodies in all flow cytometry assays
Best taken on an empty stomach to speed up the absorption
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