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J Neuroimmunol (2022) 362:577765
Giralt, M., Lafuente, A., Pujol, F
As an effector molecule, NO participates in antimicrobial responses and cooperates with oxidative stress processes to amplify inflammatory signaling, thereby being associated with an M1-like pro-inflammatory program ( + macrophage-like state with immunoregulatory features in mouse macrophage models ( In addition to generating terminal effector molecules, Arg metabolism can also influence inflammatory transcriptional programs through metabolic-signaling coupling
Cross-contamination between vials, while unlikely with proper technique, represents a risk that increases with protocol complexity
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