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For absolute quantitation of PCR reaction of each sample, amplification was performed, in parallel, on a standard curve of circularized pIRES2 plasmid, properly quantified and, on 3 l purified end joining reaction sample using CGTGTACGGTGGGAGGTCCTA forward primer and GGTACCGTCGACTGCAGAAT reverse primer, which span the sites of enzymatic restriction

NNMT Biology, Selectivity, and Off-Target Interpretation NNMT, or nicotinamide N-methyltransferase, catalyzes the N-methylation of nicotinamide using S-adenosyl-L-methionine as the methyl donor, producing N1-methylnicotinamide and S-adenosyl-L-homocysteine

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Required storage: Refrigerate at 2-8C (36-46F) immediately after mixing