The primers and probes used for Mc4r and the internal control Ngf were as follows (53): -Mc4r Forward: atttgcagcctgctttcca, Reverse: tggagcgcgtaaaagatagtga FAM-MGB Probe: tgcggtggacaggta, -Ngf, Forward: tgcatagcgtaatgtccatgttg, Reverse: tctccttctgggacattgctatc, VIC-TAMRA Probe: acggttctgcctgtacgccgatca (all primers and probes were synthesized by Thermo Fisher Scientific Inc.)
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This protein, which eventually led to the development of BPC-157, was found to be a critical factor in the bodys ability to protect and heal the stomach lining from damage
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measure the frequencies in each response option to test for ceiling and floor effects
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