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To colorimetrically measure the total AsA (reduced AsA plus oxidized DHA) and reduced AsA levels, 100 l of the supernatant was mixed with 250 l of 0.15 M of K 2 HPO 4 buffer (containing 5 mM of EDTA, pH 7.4) in the presence of either 50 l of 10 mM of dithiothreitol (for the total AsA) or 50 l of water (for the reduced AsA)

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