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Zhou L, Ni Z, Cheng W, et al
Your body makes NAD+ from Vitamin B3 (niacin)
Aging Cell 165 , 13611374 (2016)
After serial dehydration and vitrification, the tissues were cut into 5-mm sections and subjected to hematoxylin and eosin (H&E) staining
Cell culture studies demonstrate increased expression of synaptic proteins including PSD-95, synaptophysin, and synapsin following Dihexa treatment, confirming molecular mechanisms underlying the observed synaptogenic effects
RNA was reverse-transcribed to cDNA (PrimeScript RT reagent kit, Takara Clontech) and quantitative real-time polymerase chain reaction (RT-PCR) was performed with SYBR Green protocols (Kapa Biosystems, MA, USA) and a real-time PCR detection system (Applied Biosystems 7300 Real-Time PCR system) (Rinne et al, 2017
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