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Discussion 3D ET reveals distinct spherical vesicles in Arabidopsis root cells A longstanding question regarding plant retrograde transport concerns their origin, for which two distinct models have been proposed to date: (1) retrograde transport mediates the transport of VSRs from MVBs to the TGN, and (2) retrograde transport facilitates the transport of VSRs from the TGN to the Golgi and/or the ER

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