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While Hashimotos is a common root cause of hypothyroidism, its not the only cause

However, while I will occasionally recommend such pharmaceuticals only for severe symptoms, they dont target the root of the problem

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Whatever your protein source is, youll want to load it onto the column in pretty much the same way cap the bottom, add your interacting protein or cell lysate, cap the top, and incubate with gentle agitation for around an hour