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For the maximum LDH release control group, 4 l of lysis solution (Promega, USA) was added into each well and incubated for 30 mins to initiate cell lysis prior to the next step

However, addressing weight concerns does not directly treat the underlying endometriosis pathology

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If your target dose is 250mcg, divide dose by concentration: 250mcg 2500mcg/mL = 0.1mL

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