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When labs are used, they are most commonly checked at the start of care and repeated three to six months later to assess tolerance, safety, or metabolic changes

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After the cells reached ~ 80% confluence under normal culture conditions, the medium was changed to glucose-free DMEM (Gibco) and the dish was placed in an anaerobic chamber (5% CO2, 95% N2) to induce OGD-related cell injury

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