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The limitation of standard topical GHK-Cu is skin penetration depth: without penetration-enhancing excipients or palmitoylation (as in Pal-GHK), absorption to the deeper dermal layers where collagen-producing fibroblasts reside is limited

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A Dionex UltiMate 3000 506 LC System (Thermo Scientific) and a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) operated in negative mode was used for targeted measurement

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If warfarin and coenzyme Q 10 are to be used concomitantly, blood tests to assess clotting time (prothrombin time

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