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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins
Alternatives to B12 Injections: When Injections Arent Ideal For some individuals, alternative delivery methods may be considered: Vitamin B12 Patches These are applied to the skin and claim to deliver B12 transdermally
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