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Notably, in the rd10 model of retinal degeneration, Orai1 is upregulated in Mller cells exposed to oxidative stress, colocalizing with GFAP and enhancing SOCE currents [39]

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Null mutations were made using the lambda red recombinase method to replace the open reading frame (Gebendorfer et al., 2012) with a chloramphenicol resistance cassette amplified from the pKD3 template (Datsenko and Wanner, 2000)