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39 For immunohistochemistry, the tissue sections were sequentially placed in xylene I for 15 mins, xylene II for 15 mins, anhydrous ethanol I for 5 mins, anhydrous ethanol II for 5 mins, 85% alcohol for 5 mins, and 75% alcohol for 5 mins, and then were finally washed using distilled water

Published studies in animal models have reported effects on fibroblast activity the cells responsible for producing collagen and structural proteins as well as collagen organization and tissue remodeling signals

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