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This dual pathway activation allows for investigation of potential synergistic effects between GHRH and ghrelin signaling systems, providing opportunities to study how these complementary mechanisms may work together to optimize growth hormone release patterns

For example, variant X(GD_L)c.3 (later renamed X(GD_L)f) had a catalytic efficiency of 0.004 M 1 s 1 , whereas the parent X(GD_L)c that lacked reversion and engineering showed no detectable activity on the FRET substrate (Fig

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