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Introduce 20 mM IAA, incubate in darkness for 30 min, and mix each sample with 200 L of 8 M urea before transferring to filters
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Notably, PC3 cells were less sensitive to the combination treatment than LAPC-4 cells, which exhibited approximately 100 times lower survival under identical conditions [125]
Presented for research literature review only Grattagliano I et al
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