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The complete LDH substrate solution, containing the NBT solution, 50 units/ml diaphorase from Clostridium kluyveri (Sigma Aldrich) and 10 mg/ml 3-Acetylpyridine adenine dinucleotide (APAD, Sigma Aldrich) was added to the parasites
Two samples were prepared under two conditions ( Figures 1A, B ) and subjected to sequential immunoprecipitation with antibodies specific for HLA-DP (B721), pan-HLA-II (IVA12) to enrich for HLA-DQ and HLA-DR, and pan-HLA-I (W6/32)
Despite growing interest, it is important to understand that GHK-Cu is not a standalone solution or a replacement for conventional medical or dermatological treatments
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