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Reynolds, MS, PhD, Kimberly M
Western blot For the western blot in Figure 7C, we grew yeast transformants in -Leu -Trp liquid media and prepared samples by boiling yeast in sample buffer (60mM Tris pH 6.8, 25% glycerol, 2% SDS, 0.1% bromophenol blue with 14 mM beta-mercaptoethanol or 100 mM DTT)
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