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In this case, ATO was added after the first 24 hours of treatment with MTX and the number of viable cells was measured 24 hour later

FASEB J 15:11811186 Morgan MT, Nakanishi Y, Kroll DJ, Griset AP, Carnahan MA, Wathier M, Oberlies NH, Manikumar G, Wani MC, Grinstaff MW (2006) Dendrimer-encapsulated camptothecins: increased solubility, cellular uptake, and cellular retention affords enhanced anticancer activity in vitro

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