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The V3-V4 hypervariable region of the 16S rRNA gene was amplified for microbial community analysis
The six groups included (i) control group, (ii) stress group, (iii) group with DSIP one hour before stress experiments, (iv) DSIP 24 hours before stress experiments, (v) DSIP one hour before the last stress experiment and (vi) DSIP 24 hours before the last stress experiment
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Except for the EMs group ( n = 10), the three other groups were intraperitoneally injected with iron dextran (250 g/g, Shanghai Yuanye Bio-Technology Co., Ltd, China, #9004-66-4) every 2 days for 3 consecutive weeks
Strassburg CP, Oldhafer K, Manns MP, Tukey RH
