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The crude viral lysate was initially concentrated by a brief two-tier CsCl gradient centrifugation for 3 h and then the vector fraction was dialyzed in MHA buffer (3.3 mM morpholinoethanesulfonic acid [MES], 3.3 mM HEPES [pH 7.5], 3.3 mM sodium acetate)
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Se deficiency negatively impacts immune cells during activation, differentiation, and proliferation through redox signaling, oxidative burst, calcium flux, and the subsequent effector functions of immune cells (Huang et al., 2012
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