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After 48 h, the measurement was started by switching from DMEM medium to preheated glucose starvation minimal medium (140 mM NaCl, 5 mM KCl, 1 mM of MgCl 2 , 2 mM of CaCl 2 , 20 mM HEPES, pH 7.4 adjusted with NaOH) with 10% FCS

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Such instabilities may compromise the integrity of the vesicular structure, resulting in premature drug leakage and uncontrolled release kinetics

This is very important because cardiovascular disease continues to be the leading cause of death among people with Type 2 diabetes, and furthermore, Type 2 diabetes is a leading risk factor contributing to a recent rise in heart disease and stroke, said Amit Khera, M.D., M.Sc., FAHA, the volunteer chair of the American Heart Associations Advocacy Coordinating Committee and recipient of the Associations 2025 Chairmans Award