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Samples were then subjected to enzymatic digestion with trypsin/LysC (Preomics

As GLP-1 binds to GLP-1 receptors expressed on pancreatic cells, the receptors couple to G-protein subunits and activate adenylate cyclase, which increases the production of cAMP from ATP

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Therefore, the release rate R at different time points can be calculated as R = (m0 - mx) / m0 * 100%

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