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The cDNA was then fourfold diluted and used as a template in quantitative PCR with IQ SYBR Green Supermix (Bio-Rad) on a CFX96 instrument (Bio-Rad) using a 3-step protocol

Additionally, nanoparticles also address common issues associated with conventional ocular formulations, such as eye irritation and discomfort

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Starting Dose 20 units (0.6mg) Form Injectable vial (5mL, 3mg/mL, 15mg total) Dosing Protocol 20 units subcutaneous injection daily, Monday to Friday

Throughout both the noise exposure and the silent period, the animals were monitored every 15 min and returned to their cages afterward

Furthermore, we investigated cellcell fusion using the split green fluorescent protein (GFP) technique 31,32