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In the GTP-bound form, Rheb reportedly contributes to the activation of mTORC1 on the surface of lysosomes, and this process is disturbed by the activation of tuberous sclerosis complex (TSC) 67

Contrary to its limited role in feeding behavior, ghrelin is a key regulator of glucose homeostasis

After deparaffinization and hydration, the sections were treated with heated citrate buffer (pH 6.0), 0.3% H 2 O 2, and blocked with 3% BCA successively, then incubated with primary antibody at 4 C overnight and biotinylated secondary antibody

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