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Then, water was replaced by a solution containing individual PAMPs (100 nM flg22, 50 g/mL chitin, or 2 g/mL SCFE), 100 M luminol (123072, Sigma), and 20 g/mL HRP (HY-125859, MCE), and luminescence was measured in a microplate reader (PerkinElmer) for 60 or 90 min

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