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Once cellular lysis had occured, 50L of supernatant was used for derivatisation with the fluorescent dye SBD-F, as described by + formate 50mM pH 2.9 containing 3% methanol
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Coffee enemas stimulate the production of glutathione
( b ) Same as (a), but S-phase was assessed by BrdU incorporation (n = 3 technical replicates representative from 2 independent experiments)
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